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Purification of rat kidney branched-chain oxo acid dehydrogenase complex with endogenous kinase activity

R Odessey
Biochemical Journal Apr 15, 1982, 204 (1) 353-356; DOI: 10.1042/bj2040353
R Odessey
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Abstract

A method was devised to purify branched-chain oxo acid dehydrogenase (BCOAD) from rat kidney which retains endogenous kinase activity. Incorporation of 32P into purified enzyme parallels the time course of enzyme inhibition by ATP. Phosphorylation occurs on a serine residue(s) of the 46000-mol.wt. subunit of the enzyme complex. Endogenous phosphatase activity is not present after purification, and added pyruvate dehydrogenase phosphate phosphatase does not re-activate BCOAD or liberate 32P from previously labelled enzyme. These results demonstrate that BCOAD can be regulated by an endogenous protein kinase and that the phosphorylation-cycle enzymes regulating BCOAD appear to be distinct from those associated with pyruvate dehydrogenase complex.

  • © 1982 London: The Biochemical Society
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April 1982

Volume: 204 Issue: 1

Biochemical Journal: 204 (1)
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Purification of rat kidney branched-chain oxo acid dehydrogenase complex with endogenous kinase activity
R Odessey
Biochemical Journal Apr 1982, 204 (1) 353-356; DOI: 10.1042/bj2040353
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Purification of rat kidney branched-chain oxo acid dehydrogenase complex with endogenous kinase activity
R Odessey
Biochemical Journal Apr 1982, 204 (1) 353-356; DOI: 10.1042/bj2040353

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