Degradative rates of small basic non-glycosylated proteins are preferentially enhanced in rat liver cytosol during severe streptozotocin-induced diabetes. Synthetic rates of these classes of proteins are not selectively enhanced in diabetes, so small basic non-glycosylated proteins should be depleted from liver cytosol as a consequence of this disease. To test this hypothesis, proteins were analysed from normal animals, from diabetic animals receiving insulin and from diabetic animals after insulin withdrawal for 3 days. The proteins were separated according to subunit molecular weight by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, according to isoelectric point by isoelectric focusing and according to carbohydrate content by affinity chromatography with concanavalin A linked to agarose. Severe uncontrolled diabetes is associated with the predicted depletion of small basic non-glycosylated proteins from liver cytosol. The preferential degradation and loss of these protein classes may be of considerable physiological importance to the animal.
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July 1981
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Research Article|
July 15 1981
Selective depletion of small basic non-glycosylated proteins in diabetes
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1981 London: The Biochemical Society
1981
Biochem J (1981) 198 (1): 149–157.
Citation
F C Samaniego, F Berry, J F Dice; Selective depletion of small basic non-glycosylated proteins in diabetes. Biochem J 15 July 1981; 198 (1): 149–157. doi: https://doi.org/10.1042/bj1980149
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