Microsomal fraction was prepared by ultracentrifugation of homogenates of cortical tissue from bovine brains. The preparation displayed ATPase (adenosine triphosphatase) activity in the presence of Mg2+ (6.4μmol of Pi/h per mg of protein) and Ca2+ (3.4μmol of Pi/h per mg of protein). Kinetic analysis of the activation of the enzyme preparation by Ca2+ resulted in the demonstration of two apparent Km values for Ca2+ (6.0×10−8m and 1.2×10−6m). Treatment of the microsomal membranes with Triton X-100 resulted in solubilization of the ATPase, though with some loss of activity. The solubilized microsomal proteins were incorporated into liposomes. By incubation of the liposomes in media containing 45Ca2+ an ATP-dependent uptake of Ca2+ was demonstrated. The solubilized preparation was subjected to preparative isoelectric focusing in granulated gel beds. Two distinct peaks of Mg2+- and Ca2+-dependent ATPase activity were observed at pH4.8 (peak 4.8) and at pH6.3 (peak 6.3). The material isolated in peaks 4.8 and 6.3 was focused in polyacrylamide gel with pH gradients. The material corresponding to peak 4.8 consisted of a single protein, whereas peak 6.3 contained one major and at least one minor protein. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis confirmed these results and indicated that the major component of peak 4.8 and the protein of peak 6.3 both had a molecular weight of 105000. The material in peaks 4.8 and 6.3 was assayed for ATPase activity in the presence of various concentrations of Ca2+. Kinetic analysis of the results for peak 4.8 demonstrated an apparent Km value for Ca2+ of 4.1×10−8m. The enzyme isolated at pH6.3 had an apparent Km value of 3.8×10−6m. However, when the material from peak 4.8 was incubated in the presence of 1mm-Mg2+ the ATPase could not be activated by Ca2+.
- © 1979 London: The Biochemical Society