1. A new assay system is described for monoglyceride acyltransferase (acylglycerol palmitoyltransferase, EC 2.3.1.22) in which palmitoyl-CoA is generated from palmitoyl-(-)-carnitine. 2. With the microsomal fraction from homogenates of guinea-pig intestinal mucosa, the Vmax. of this enzyme decreased with different acyl acceptors in the order 2-monopalmitoylglycerol>2-hexadecylglycerol>rac-1-monopalmitoylglycerol. 3. There were highly significant correlations between the monoglyceride acyltransferase activity as measured with these three substrates. This demonstrates that each of these substrates can be used to measure the same enzyme activity. 4. The advantages of using generated palmitoyl-CoA with 2-hexadecylglycerol and rac-1-monopalmitoylglycerol as model substrates for this enzyme are discussed.
Skip Nav Destination
Article navigation
August 1974
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
August 01 1974
A new assay procedure for monoglyceride acyltransferase
Verena J. Short;
Verena J. Short
1Department of Biochemistry, The Medical School, University of Nottingham, Nottingham NG7 2RD, U.K.
Search for other works by this author on:
David N. Brindley;
David N. Brindley
1Department of Biochemistry, The Medical School, University of Nottingham, Nottingham NG7 2RD, U.K.
Search for other works by this author on:
Raymond Dils
Raymond Dils
1Department of Biochemistry, The Medical School, University of Nottingham, Nottingham NG7 2RD, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© 1974 London: The Biochemical Society
1974
Biochem J (1974) 141 (2): 407–411.
Citation
Verena J. Short, David N. Brindley, Raymond Dils; A new assay procedure for monoglyceride acyltransferase. Biochem J 1 August 1974; 141 (2): 407–411. doi: https://doi.org/10.1042/bj1410407
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.