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Nitrogen metabolism in the isolated perfused rat liver. Nitrogen balance, redox state and rates of proteolysis

R. Parrilla, M. N. Goodman
Biochemical Journal Mar 01, 1974, 138 (3) 341-348; DOI: 10.1042/bj1380341
R. Parrilla
Joslin Research Laboratory, Department of Medicine, Harvard Medical School, Peter Bent Brigham Hospital, and the Joslin Diabetes Foundation Inc., Boston, Mass. 02215, U.S.A., and Department of Metabolism, Instituto G. Marañon, C.S.I.C., Madrid, Spain
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M. N. Goodman
Joslin Research Laboratory, Department of Medicine, Harvard Medical School, Peter Bent Brigham Hospital, and the Joslin Diabetes Foundation Inc., Boston, Mass. 02215, U.S.A., and Department of Metabolism, Instituto G. Marañon, C.S.I.C., Madrid, Spain
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Abstract

Nitrogen balances were measured in isolated perfused rat livers in the presence and absence of nitrogen donors. In all instances the balance apparently was incomplete. The expression [alanine][α-oxoglutarate]/[pyruvate][glutamate] remained fairly constant under the metabolic conditions studied, indicating that it may be at near-equilibrium. The source of the extra nitrogen seems to be derived from increased hepatic proteolysis. The addition of a nitrogen donor to the perfusate arrested proteolysis, as did the addition of pyruvate. The free mitochondrial [NAD+]/[NADH] ratio, calculated from the glutamate dehydrogenase and β-hydroxybutyrate dehydrogenase reactants, showed similar values and exhibited parallel changes under most metabolic situations studied. These results suggest that, under the reported experimental conditions, both dehydrogenases share a common mitochondrial NAD pool. Glutamate dehydrogenase plays an important role in hepatic nitrogen metabolism in vivo.

  • © 1974 London: The Biochemical Socity
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March 1974

Volume: 138 Issue: 3

Biochemical Journal: 138 (3)
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Nitrogen metabolism in the isolated perfused rat liver. Nitrogen balance, redox state and rates of proteolysis
R. Parrilla, M. N. Goodman
Biochemical Journal Mar 1974, 138 (3) 341-348; DOI: 10.1042/bj1380341
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Nitrogen metabolism in the isolated perfused rat liver. Nitrogen balance, redox state and rates of proteolysis
R. Parrilla, M. N. Goodman
Biochemical Journal Mar 1974, 138 (3) 341-348; DOI: 10.1042/bj1380341

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