1. The phosphomannan of Saccharomyces cerevisiae was released by Pronase digestion of cell walls and isolated by chromatography on DEAE-cellulose or by precipitation with borate–Cetavlon solutions. Mannose and phosphorus were present in the molar ratio 18:1 and the phosphate groups were in the diester form. 2. Hydrolysis with acid gave mannose 6-phosphate. Under mild acid conditions (autohydrolysis) the phosphate groups were converted into the monoester form, mannose was released and the molecular size of the phosphomannan was substantially decreased. 3. Hydrolysis with alkali also gave a monoester phosphate and a similar decrease in molecular weight. Under mild alkaline conditions the serine and threonine content of the phosphomannan was decreased by about 80%. The phosphate content was not altered. 4. Treatment with 40% (v/v) HF removed 70% of the phosphorus from the phosphomannan with no detectable decrease in molecular weight. 5. Periodate oxidation gave an oxophosphomannan from which 80% of the phosphorus was eliminated under mild alkaline conditions. 6. The properties of the phosphomannan are consistent with a structure in which the phosphate groups are located on the outside of the molecule and link C-1 of a terminal mannose unit with C-6 of another mannose unit, which is in turn attached to the polysaccharide backbone of the molecule. 7. The implications of this structure are discussed in relation to flocculation.
Skip Nav Destination
Article navigation
September 1972
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
September 01 1972
A study of the phosphate linkages in phosphomannan in cell walls of Saccharomyces cerevisiae
T. N. Cawley;
T. N. Cawley
1Department of Biochemistry, University College, Dublin 2, Irish Republic
Search for other works by this author on:
M. G. Harrington;
M. G. Harrington
1Department of Biochemistry, University College, Dublin 2, Irish Republic
Search for other works by this author on:
R. Letters
R. Letters
2Research Laboratory, Arthur Guinness Son and Co. (Dublin) Ltd., St. James's Gate, Dublin 8, Irish Republic
Search for other works by this author on:
Publisher: Portland Press Ltd
© 1972 London: The Biochemical Society
1972
Biochem J (1972) 129 (3): 711–720.
Citation
T. N. Cawley, M. G. Harrington, R. Letters; A study of the phosphate linkages in phosphomannan in cell walls of Saccharomyces cerevisiae. Biochem J 1 September 1972; 129 (3): 711–720. doi: https://doi.org/10.1042/bj1290711
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.