1. Mitochondria were extracted from 4-day-old pea cotyledons and purified on a sucrose density gradient. 2. Microbiological assay of the purified mitochondrial fraction with Lactobacillus casei (A.T.C.C. 7469), Streptococcus faecalis (A.T.C.C. 8043) and Pediococcus cerevisiae (A.T.C.C. 8081) revealed a discrete pool of conjugated and unconjugated derivatives of tetrahydropteroylglutamic acid. 3. Solubilization and chromatographic studies of the mitochondrial fraction demonstrated the presence of formylated and methylated derivatives, 10-formyltetrahydropteroylmonoglutamic acid, 5-formyltetrahydropteroylmonoglutamic acid and 5-formyltetrahydropteroyldiglutamic acid being the major derivatives present. 4. The principal mitochondrial pteroylglutamates were labelled when dry seeds were allowed to imbibe [2-14C]pteroylglutamic acid and 5-[methyl-14C]-methyltetrahydropteroylmonoglutamic acid. 5. The ability of isolated mitochondria to catalyse oxidation and reduction of tetrahydropteroylglutamic acid derivatives was demonstrated in feeding experiments in which [14C]formaldehyde, [3-14C]serine, sodium [14C]formate, 5-[methyl-14C]methyltetrahydropteroylmonoglutamic acid or [2-14C]-glycine served as C1 donor. In addition,14C was incorporated into free amino acids related to C1 metabolism.
Skip Nav Destination
Article navigation
June 1972
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Research Article|
June 01 1972
Localization and interconversion of tetrahydropteroylglutamates in isolated pea mitochondria
M. T. Clandinin;
M. T. Clandinin
1Department of Botany, University of Alberta, Edmonton 7, Alberta, Canada
Search for other works by this author on:
E. A. Cossins
E. A. Cossins
1Department of Botany, University of Alberta, Edmonton 7, Alberta, Canada
Search for other works by this author on:
Publisher: Portland Press Ltd
© 1972 London: The Biochemical Society
1972
Biochem J (1972) 128 (1): 29–40.
Citation
M. T. Clandinin, E. A. Cossins; Localization and interconversion of tetrahydropteroylglutamates in isolated pea mitochondria. Biochem J 1 June 1972; 128 (1): 29–40. doi: https://doi.org/10.1042/bj1280029
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.