1. Aldolase isoenzymes from guinea-pig cerebral cortex were partially purified and separated by ammonium sulphate fractionation and chromatography on DEAE-cellulose. 2. Each purified isoenzyme was shown to be virtually uncontaminated with other forms by starch-gel electrophoresis. The quantitative distribution of the isoenzymes was: I, 6·2%; II, 5·2%; III, 15·3%; IV, 25·7%; V, 33·3%. 3. The pH optima for the five separated isoenzymes were similar; all were in the range pH7·5–8·0. Values for pKa (6·31–6·55) and pKb (9·45–9·59) were calculated from the data and suggested the involvement of histidine and lysine residues. 4. The stabilities of the isoenzymes were shown to be I=II>III>IV>V at pH4·4 in order of decreasing stability and are discussed in terms of subunit structure. 5. The substrate activity ratios (fructose 1,6-diphosphate/fructose 1-phosphate) were measured and all were in the range 12–44.
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Research Article|
May 01 1969
The separation, partial purification and some properties of isoenzymes of aldolase from guinea-pig cerebral cortex
P. C. Nicholas;
P. C. Nicholas
1Department of Biochemistry, Institute of Psychiatry (British Postgraduate Medical Federation, University of London), De Crespigny Park, London S.E.5
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H S Bachelard
H S Bachelard
1Department of Biochemistry, Institute of Psychiatry (British Postgraduate Medical Federation, University of London), De Crespigny Park, London S.E.5
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Publisher: Portland Press Ltd
© 1969 The Biochemical Society
1969
Biochem J (1969) 112 (5): 587–594.
Citation
P. C. Nicholas, H S Bachelard; The separation, partial purification and some properties of isoenzymes of aldolase from guinea-pig cerebral cortex. Biochem J 1 May 1969; 112 (5): 587–594. doi: https://doi.org/10.1042/bj1120587
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