1. The specific activity of the enzyme palmitoyl-CoA–l-glycerol 3-phosphate palmitoyltransferase (EC 18.104.22.168) in the mammary tissue of guinea pigs has been shown to increase 37-fold at parturition. 2. Increases also occur in tissue concentrations of glycerol 3-phosphate, CoA and free fatty acid, but not in that of acid-insoluble CoA. 3. The isolation and fatty acid composition of plasma triglyceride and of mammary-tissue free fatty acid, diglyceride and triglyceride are described. 4. The findings are discussed in relation to the regulation of milk fat synthesis.
- © 1967 The Biochemical Society