Listen to Daniel Madsen, first author on a study by Thomas Bugge and colleagues, discussing his paper, in which TMPRSS13 (transmembrane protease, serine 13), a type II transmembrane serine protease of unknown function, is shown to be highly expressed in many multilayered epithelia lining body cavities. The paper also describes how this protease is important for skin development.
Listen to Professor Jung Weon Lee discussing his latest research, in which the suppression of TM4SF5 (transmembrane 4 L six family member 5) is shown to disturb integrin α5-related signalling and muscle development in zebrafish.
Listen to Julian Stevenson (first author on the paper) and Andrew Brown (the corresponding author on the study) discussing their latest paper, in which the cholesterol biosynthetic enzyme squalene mono-oxygenase is shown to be stabilized by unsaturated fatty acids, which blunt its polyubiquitination by the E3 ubiquitin ligase MARCH6.
Listen to Kristen Funk, first author on the paper by Austin Yang, Jeff Kuret and colleagues, discussing her latest paper, in which lysine methylation has been identified as an endogenous post-translational modification of tau protein in human brain and a modulator of aggregation propensity.
Listen to Adrienne Sullivan, first author on a study by Murray Whitelaw and colleagues, discussing her research, in which they have assessed a number of human variants of the transcription factor SIM1 with reduced activity to determine that impaired function is frequently due to defects in dimerization with the essential partner protein ARNT2 (aryl hydrocarbon nuclear translocator 2).
Listen to Torsten Wundenberg, first author on a study by Georg Mayr and colleagues, discussing his research, in which inositol hexakisphosphate (InsP6) kinases are identified as InsP6 phosphorylating and dephosphorylating enzymes synthesizing either 5PP-InsP5 or Ins(2,3,4,5,6)P5.
Listen to Jean-Christophe Jonas discussing his latest paper, in which nutrient stimulation acutely reduces mitochondrial, but not cytosolic/nuclear, glutathione redox potential (EGSH) in insulin-secreting β-cells under control conditions.
Listen to Carol (Xiaoying) Koh-Stenta (first author on the paper) and Jeffrey Hill (the corresponding author) discussing their latest work, in which they have performed comprehensive characterization of an archetypal member of the PRDM family, PRDM9, and demonstrated its chemical tractability in a compound library screen. Significantly, their results have shown that PRDM9 has broader activities than was previously recognized.
Listen to Yoel Klug (first author on the study) discussing the latest open access paper by Yechiel Shai and co-workers, in which they have shown that sphinganine lipidated peptides affect membrane fusion, modulate the membrane and disrupt protein assembly. Furthermore, their findings may aid in deciphering the function of DHSM (dihydrosphingomyelin) in biological membranes.
Listen to Sona Pandey describe her latest research which establishes that specific plant Gα proteins are functional in the receptor-mediated pheromone-response pathway in yeast and that the subtle biochemical differences in their activity are physiologically relevant.
Listen to Xin Qi describe his study which identified a key role of Drp1 in the induction of mitochondria-related necrosis under conditions of oxidative stress in vitro and in vivo, suggesting that Drp1 might be a useful target to combat necrosis in human disease.
Listen to André Silva describe his study of human serum transferrin, which is susceptible to modification under elevated glycaemic conditions, such as those encountered in diabetes mellitus. This study of transferrin glycation shows that key amino acid residues undergo glycation, inducing structural alterations that compromise its function as an iron-binding protein.
Listen to Griselda Hernández describe her latest paper on how the FK1 domain of FKBP51 exhibits microsecond-millisecond conformational dynamics in the β3 bulge and the β4-β5 loop, known sites of protein signalling interactions.
Listen to Mark Rider describe his latest research of a small-molecule AMPK (AMP-activated protein kinase) activator that efficiently activates AMPK and stimulates glucose uptake and fatty acid oxidation in skeletal muscle.
Listen to Adolfo Saiardi (corresponding author) and Bernadett Kolozsvari (first author on the paper) discussing their latest work, in which they show how the inositol phosphates IP5 and IP6 induce a specific DAPI fluorescence, whereas lower phosphorylated forms do not. They also demonstrate that the fluorescence properties of DAPI-IP5 and DAPI-IP6 complexes allow the easy assessment of the amounts and enzymatic conversion of these two important cellular messengers.
Listen to Lih-Wen Deng (corresponding author) and Zheng-Wei Lee (first author on the paper) discussing their latest research, in which they show how the cytoprotective role of CSE (cystathionine γ-lyase) is attributable to the ability of H2S produced by CSE to increase the GSH concentration and to preserve glutathione status, thereby highlighting the importance of the CSE/H2S system in cellular redox.
Listen to Vanessa Johanssen (first author on the paper) and Steve Collins (corresponding author on the study) discussing their latest work, in which C-terminal fragments generated through α- and β-cleavage of cellular prion protein appear to harbour different pathogenic potential for host cells. Most interestingly, α-cleavage produces a C-terminal fragment that is resistant to folding into soluble β-strand-rich toxic isoforms.
Fungal polyglycine hydrolases cleave multiple specific peptide bonds in the linker domains of targeted plant chitinases. These proteases somehow recognize polyglycine linkers in targeted chitinases. Listen to Todd Naumann discussing his latest paper in which polyglycine hydrolases secreted by Pleosporineae fungi have been shown to target the linker region of plant class IV chitinases.
Listen to Swee Kim Ang, first author on a paper written by Hui Lu and colleagues, discussing his latest research in which they show that, although both shuttle cysteine residues of Erv1 (a thiol enzyme that is an essential component of the mitochondrial MIA pathway) are required for its function, they play complementary but distinct roles to ensure rapid turnover of active enzyme.
Listen to Rupesh Paudyal (first author on the paper) and the corresponding author, Alison Baker, discussing their latest paper in which a small molecule inhibitor of protein trafficking and the gravitropic response in Arabidopsis thaliana (TENin1) has been characterized in detail.
Listen to Carlos Reguenga and Ricardo Soares-dos-Reis (first author on the paper) discussing their latest work in which the homeodomain transcription factor PRRXL1 has been shown to acquire different phosphorylation profiles at distinct dorsal spinal cord and dorsal root ganglion developmental stages.
Listen to Tatsuo Adachi discuss the study of Niemann-Pick disease type C2 protein inducing triglyceride accumulation via the activation of AMPK independently of its cholesterol-binding capacity in the silkworm.
Listen to Dr Susanne Müller-Knapp discussing her latest paper, which is freely available to all readers, in which her group has determined crystal structures of GAK (cyclin G-associated kinase) in complex with specific single-chain antibodies known as 'nanobodies' to reveal the domain plasticity of this kinase and its unusual activation segment architecture.
Listen to Liz Ledgerwood discussing her latest paper in which the peroxidase activity of human and mouse cytochrome c residue 41 variants has been analysed to demonstrate that stimulation of peroxidase activity can occur without prior loss of Fe-Met80 co-ordination or partial unfolding.
Listen to Ruth Palmer (corresponding author on the study) and Yasuo Yamazaki discussing their latest paper in which they have identified a role for the PA (protease-associated) domain of the RNF13/RNF167 ubiquitin ligases, showing that it is both sufficient and required for endosomal localization.
Listen to Jacky Ngo discussing his latest paper in which the kinase SRPK2 (serine/arginine protein kinase 2) has been shown to bind and phosphorylate different substrates, including SRSF1 (serine/arginine splicing factor 1) and SR-like protein acinusS, by utilizing a docking groove that is unique to SRPKs.
Listen to Barry Lentz (corresponding author on the paper) and Rinku Majumder discussing their latest paper in which FRET (fluorescence resonance energy transfer) has been used to monitor the structure of Factor Xa alone and complexed with Factor Va.
Listen to James Murphy being interviewed by a co-author, Peter Czabotar, about his paper in which crystal structure and mutagenesis studies of the human pseudokinase MLKL (mixed lineage kinase domain-like), an essential necroptosis pathway molecular switch, have provided insights into how pseudokinase domains accomplish nucleotide binding despite lacking the conventional ATP-binding/catalytic motifs typical of active protein kinases.
Listen to Jason McArthur discussing his latest paper in which, on an examination of the roles of bacterial and host-derived cofactors in streptokinase-mediated plasminogen activation, his group has found that phenotypic streptokinase variation functionally underpins a pathogenic mechanism whereby streptokinase variants differentially focus plasminogen activation, leading to specific niche adaption within the host.
Listen to Bill Plaxton and the first author on the study, Allyson Hill, discussing their latest paper, in which inhibitory Ser451 phosphorylation of bacterial-type PEPC (phosphoenolpyruvate carboxylase) subunits of the Class-2 PEPC hetero-octameric complex of developing castor beans has been shown to be catalysed by a Ca2+-dependent protein kinase.
Listen to Yasunori Yamamoto, first author on a paper by Toshiaki Sakisaka and colleagues, discussing his latest paper, in which Arl6IP1, an anti-apoptotic regulator, has been characterized in the context of a protein harbouring the reticulon-homology domain.
Listen to James Murphy being interviewed by Jeff Babon (a co-author) on his recent paper, in which a thermal-shift assay has been employed to characterize the nucleotide-binding properties of 31 diverse pseudokinase domains. The authors discuss how their data support the notion that pseudokinases predominantly serve non-catalytic functions in cellular signalling.
Listen to Monika Slomińska Wojewódzka discussing her latest paper in which the role of the endoplasmic reticulum chaperone protein EDEM2 in ricin cytotoxicity and its retrotranslocation from the endoplasmic reticulum to the cytosol have been investigated.
Listen to Ariel Stanhill discussing his latest paper in which AIRAPL (arsenite-inducible RNA-associated protein-like protein) has been identified as a p97 adaptor on the ER membrane involved in translocation processes into the ER.
Listen to Ben Bikman discussing his paper in which his group have shown that ceramide 'forces' mitochondrial fission, which is an essential mediator of ceramide-induced metabolic disruption in skeletal muscle.
Listen to Xin'an Lu, first author on a paper by Yongzhang Luo and colleagues, discussing his work in which for the first time a client kinase has been shown to directly regulate Hsp90 activity: a novel level of regulation for the Hsp90 chaperone machinery.
Listen to Natasha Boase, joint first author on a paper written by Sharad Kumar and colleagues, discussing her work in which it has been demonstrated for the first time that the ubiquitin ligase Nedd4-2 regulates voltage-gated sodium channel activity in cortical neurons, specifically in response to elevated intracellular Na+ concentrations.
Listen to Angela Svendsen, first author on the paper (corresponding authors: Mary Horne and Pierre De Meyts) discussing her latest work in which it has been demonstrated that regulation of cyclin G2 provides a key mechanism whereby insulin, insulin analogues and IGF-I (insulin-like growth factor 1) stimulate cell proliferation.
Listen to Agueda Rostagno discussing her latest paper in which the contributions of the post-translational isoaspartate modification and the D23N mutation to the aggregation/oligomerization and cell toxicity properties of amyloid β in Alzheimer's disease have been highlighted.
Listen to Ilia Voskoboinik being interviewed by Dr Jane Oliaro (a colleague) about his latest paper in which the molecular mechanism that governs the binding of an essential immune protein, cytotoxic lymphocyte perforin, to the plasma membrane of a target cell, and the subsequent initiation of pore formation, has been resolved.
Listen to Christine E. Schaner Tooley discussing her latest paper in which the existence of a second mammalian N-terminal methyltransferase (NRMT2) has been verified that is shown to have a different catalytic activity from that of its previously identified homologue (NRMT1).
Listen to Dimitri Svistunenko (joint corresponding author with Jonathan Worrall) discussing his latest paper in which a mechanism of multiple radical formation is proposed to account for the enhanced apoptotic activity of the G41S mutation in cytochrome c.
Listen to Francis Chan discussing his paper in which RIP1 (receptor-interacting serine/threonine protein kinase 1) is regulated by positive as well as inhibitory phosphorylation; furthermore, a gain-of-function RIP3 mutant can partially bypass the requirement of RIP1 in tumour necrosis factor-induced programmed necrosis.
Listen to Nicholas Lim discussing his recent paper in which DiOHF (3',4'-dihydroxyflavonol) inhibition of Ca2+/calmodulin-dependent protein kinase II and attenuation of MKK3/6 (MAPK kinase 3/6)/→p38MAPK and MKK4/7→JNK (c-Jun N-terminal kinase) signalling are shown to be required for the protective effects of DiOHF against stress stimuli and myocardial ischaemia/reperfusion injury.
Listen to Manoj Menon discussing his paper in which the endoplasmic reticulum-associated ubiquitin-conjugating enzyme Ube2j1 is shown to be a novel substrate of MK2 (MAPKAP kinase-2) involved in MK2-mediated tumour necrosis factor α production.
Listen to Ambuj Kushwaha discussing his paper, co-written with Anne Grove, in which Mycobacterium smegmatis Ku protein, which is required for DNA double-strand-break repair, can translocate from DNA ends to internal DNA sites. He discusses how it can also bind DNA without free ends via its C-terminal lysine-rich extension.
Listen to David Vaux discussing his paper in which studying seeding in an interfacial context (i.e. the air-water interface) is shown to be crucial to understanding amyloidogenesis in vivo.
Listen to Jean-Marc Taymans and Jeremy Nichols talking about their latest work on the identification of protein phosphatase 1 as the physiological LRRK2 phosphatase, which is responsible for LRRK2 dephosphorylation observed in Parkinson's disease mutant LRRK2 and after LRRK2 kinase inhibition.
Listen to Rongjia Zhou discussing his paper in which his group have identified a molecular mechanism that VHL (von Hippel-Lindau) tumour suppressor and UXT (ubiquitously expressed transcript) regulate androgen receptor signalling.
Listen to Ying Lai discussing his paper in which a single-vesicle-fusion assay has been used to dissect the function of the Syt1 linker region of synaptotagmin 1. Their results suggest that the Syt1 linker region might have some capacity to extend for docking and fold to facilitate pore opening.
Listen to Kajal Kanchan, first author on the paper by László Fésüs and colleagues, discussing her latest work in which Val224 has been identified as a crucial amino acid residue that increases transglutaminase 2 activity by modulating its calcium-binding affinity, thereby enabling transamidation reactions inside cells.
Listen to Christina Schluepen, first author on a paper by Sabrina Liberatori and colleagues, talking about their latest work in which the importance of the Csa family of staphylococcal specific antigens as novel vaccine candidates is highlighted.
Listen to Kenneth Cheng, first author on a paper by Aimin Xu and colleagues, discussing their work in which it has been shown that APPL1 ubiquitination blocks insulin-evoked membrane localization of the APPL1-Akt complex, leading to impaired insulin actions on Akt activation and suppression of hepatic glucose production.
Listen to Alberto Bartolomé and Carlos Guillén discussing individually their latest paper in which autophagy has been shown to be an essential mechanism for the maintenance of osteoblast viability and function in a high-glucose environment.
Listen to Mark Rider discussing his latest paper in which PIKfyve activity is shown to be required for the stimulation of skeletal muscle glucose uptake by contraction/AMPK activation. Also read the commentary on this paper that has been written by James Lally and Greg Steinberg.
Listen to Jessica Fetterman, first author on the paper (corresponding authors: Danny Welch and Scott Ballinger) discussing her work in which mtDNA has been shown to serve as a major determinant of cardiac bioenergetics, cellular oxidant production and susceptibility to cardiac stress, independent of the nuclear genome.
Listen to Alessio Peracchi discussing his group's latest paper, an accelerated publication in which they have identified the aminodeoxychorismate lyase from Plasmodium falciparum. This enzyme participates in the biosynthesis of folate, and is a putative novel target for antimalarial therapy.
Listen to Kathryn Taylor, the corresponding author on the paper, in a discussion with a couple of her co-authors (Peter Kille and Christer Hogstrand) on how they have demonstrated a novel mechanism for the ability of cellular zinc to drive cell detachment and migration, with implications for breast cancer spread.
Listen to Ruediger Schweigreiter discussing his latest paper in which the neurite outgrowth inhibitor Nogo-A is closely involved in the neuronal response to hypoxic and oxidative stress.
Listen to Carol Troy discussing her latest paper in which it has been demonstrated that caspase-2 is essential for c-Jun transcriptional activation and Bim induction in neuron death.
Listen to Hui Lu discussing her latest paper in which mitochondrial protein Mia40 has been identified and characterized as an iron-sulfur protein.
Listen to Ohara Augusto discussing her latest paper in which her group has characterized the effects of tempol, which reduces tissue injury in diverse animal models of oxidative stress and inflammation, on human superoxide dismutase 1 oxidation in vitro.
Listen to Sunghoon Kim discussing his latest paper in which the tumorigenic activity of a variant of AIMP2 (aminoacyl-tRNA synthetase-interacting multifunctional protein 2) has been controlled by a small chemical.
Listen to Aymeric Goyer discussing his work with Andrew Hanson and colleagues on the function of a group of Nudix enzymes from fungi and plants, showing that they hydrolyse oxidized forms of the enzyme cofactor thiamin diphosphate.
Listen to Leyuan Liu discussing his latest paper in which suppression of the mitochondrial protein LRPPRC enriches Beclin 1-PI3KCIII complex to activate autophagy and mitophagy.
Listen to Paul Huang discussing his latest paper in which the protein tyrosine phosphatase SHP-2 is shown to be a key signalling node downstream of the DDR2 (Discoidin domain 2) receptor.
Listen to Peter Stogios (in the lab of Alexei Savchenko) discussing his latest paper on the three-dimensional structural characterization of the aminoglycoside phosphotransferase APH(3')-Ia, in which the structural basis of inhibition mediated by three distinct protein kinase inhibitor scaffolds has also been examined.
Listen to Jianhua Zhang discussing her latest paper on how, in response to nutrient deprivation in mouse embryonic fibroblasts, Sirt3 decreases the requirement for enhanced autophagy and improves cellular bioenergetics.
Listen to Patricia Dos Santos discussing her latest paper on the cross-functionalities of Bacillus deacetylases that are involved in bacillithiol biosynthesis and bacillithiol-S-conjugate detoxification pathways.
Listen to Stephen Yarwood discussing his latest paper on novel molecular actions of the flavanoids naringenin and flavone as effective inducers of SOCS3 gene induction and suppressors of STAT3 signalling in vascular endothelial cells.
Listen to Griselda Hernandez discussing her latest paper, which is freely available to all readers, on the insights gained from an analysis of the visible conformational substates of the FK506-binding protein FKBP12.
Listen to Jonathan Clarke discussing his latest paper (with Robin Irvine) on how differences in PI5P4K isoform activity and localization are due to evolutionarily conserved structural changes.
Listen to Arindam Saha discussing his latest paper (co-first author Alla Fridman with Gerry Boss as the principal investigator) on how purine synthesis is regulated during the cell cycle by phosphoribosyl pyrophosphate and inorganic phosphate.
Listen to MengJie Hu (supervised by Marie Bogoyevitch) discussing her latest paper on how p32 protein levels are integral to mitochondrial and endoplasmic reticulum morphology, cell metabolism and survival.
Listen to Christine Winterbourn discussing her latest paper on the interaction of hyperoxidized peroxiredoxin 2 with the protein disulphide-isomerase ERp46.
Listen to Weihua Bian discussing her latest paper (co-first author Licui Sun and Longyan Yang with Junqi He as the principal investigator) on a novel mechanism of Mas receptor regulation in which its expression is modulated at the post-translational level by ubiquitination.
Listen to Bill Kobertz discussing in his latest paper the identification of co- and post-translational consensus sites in the KCNE family of K+ channel regulatory subunits in order to define the molecular determinants that affect the kinetics of N-glycosylation in type I transmembrane peptides.
Listen to Alex Wolf (first author on a paper written by Christopher Schofield, Angelika Böttger and colleagues) discussing their latest paper in which the polyserine domain of the lysyl-5 hydroxylase Jmjd6 is shown to mediate subnuclear localization.
Listen to Dan Hu (supervised by Jun Hirabayashi) discussing his latest paper on how GalNAcα1-3Galβ-specific lectins may be tailored from a multi-specific fungal galectin.
Listen to Ganesh Shankarling (supervised by Kristen Lynch) discussing his latest paper, which discloses important functional and mechanistic variations between two highly related hnRNP proteins, hnRNP L and hnRNP LL.
Listen to Barbara Munz discussing her latest paper in which skNAC (skeletal-and-heart-muscle-specific-variant-of-nascent-polypeptide-associated complex) is shown to regulate calpain activity.
Listen to Heather Melrose discussing her latest paper on ten novel monoclonal antibodies that target epitopes across the LRRK2 protein.
Listen to Peter Shepherd, Chair of the Editorial Board, providing an outline of the journal's painless publishing initiative. Read also the editorial that he has written on this topic.
Listen to Peter Shepherd discussing his recent paper, in which he describes a pathway by which changes in glucose levels can regulate β-catenin using a mechanism that involves cAMP production and the activation of PKA.